All stocks with insertions of UAS-RNAi constructs available from Bloomington are listed below. The majority are lines generated by the Transgenic RNAi Project (TRiP). The TRiP toolbox stocks used to generate TRiP RNAi transgenic lines and to assess their ability to knock down gene expression are also available from Bloomington, as are the Dickson/VDRC RNAi toolbox stocks.
Basic VALIUM vector information (for specifics, please see the TRiP site)
The TRiP group is providing constructs based on five different vectors -- VALIUM1, VALIUM10, VALIUM20, VALIUM21 and VALIUM22.
1) VALIUM1 - these are the "weakest" and very likely require the inclusion of UAS-Dicer2 for maximum effect
2) VALIUM10 - UAS-Dicer2 likely not needed; works well in the soma but usually not as well as VALIUM20
3) VALIUM20 - UAS-Dicer2 not needed as they use shRNAs; usually gives stronger effects than VALIUM10 in the soma; also works well in the germline
4) VALIUM21 and VALIUM22 - UAS-Dicer2 not needed as they use shRNAs; both contain a P transposase basal promoter and a K10 polyA for germline expression and should work better than VALIUM20 in the female germline. These do not work well, if at all, in the soma.