All available TRiP-CRISPR guide RNA lines are listed here. The insertions must be combined with a source of Cas9 to induce gene knockout or overexpression. See the TRiP-CRISPR toolbox for a list of available stocks with both Cas9 and a GAL4 driver.
There are two TRiP-CRISPR gRNA stock collections (for specifics, please see the TRiP in vivo CRISPR site):
TRiP-CRISPR Overexpression (TRiP-OE) - activate expression of the target gene in somatic and/or germline cells by crossing to a line expressing GAL4 and dCas9-VPR
TRiP-CRISPR KnockOut (TRiP-KO) - generate indels (small insertion or deletion mutations) in somatic and/or germline cells by crossing to a line expressing GAL4 and Cas9
TRiP-CRISPR lines are generated in either pCFD3 or pCFD4 vectors (Port et al., 2014) which are designed to ubiquitiously express either one or two sgRNAs, respectively. Both vectors contain attB sites for phiC31-recombination, as well as the vermillion selection marker. In pCFD3, a single sgRNA is driven by the snRNA:U6:96Ac (U6:3) promoter and in pCFD4 the sgRNAs are driven by snRNA:U6:96Aa (U6:1) and snRNA:U6:96Ac. Detailed descriptions of these vectors are available at the CRISPR Fly Design site.
The TRiP encourages the community to nominate genes for TRiP-OE and TRiP-KO production via the TRiP sgRNA Database. If use of any of the TRiP-CRISPR stocks results in data used in a publication, please cite the TRiP in your publication.
** If indicated, this insertion targets more than one gene. To see the other targets, either search this page with the stock number or click on the stock number to read the stock report.