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guide RNA stocks

All available TRiP-CRISPR guide RNA lines are listed here. The insertions must be combined with a source of Cas9 to induce gene knockout or overexpression. See the TRiP-CRISPR toolbox for a list of available stocks with both Cas9 and a GAL4 driver.

There are two TRiP-CRISPR gRNA stock collections (for specifics, please see the TRiP in vivo CRISPR site):

  1. TRiP-CRISPR Overexpression (TRiP-OE) - activate expression of the target gene in somatic and/or germline cells by crossing to a line expressing GAL4 and dCas9-VPR
  2. TRiP-CRISPR KnockOut (TRiP-KO) - generate indels (small insertion or deletion mutations) in somatic and/or germline cells by crossing to a line expressing GAL4 and Cas9

TRiP-CRISPR lines are generated in either pCFD3 or pCFD4 vectors (Port et al., 2014) which are designed to ubiquitiously express either one or two sgRNAs, respectively. Both vectors contain attB sites for phiC31-recombination, as well as the vermillion selection marker. In pCFD3, a single sgRNA is driven by the snRNA:U6:96Ac (U6:3) promoter and in pCFD4 the sgRNAs are driven by snRNA:U6:96Aa (U6:1) and snRNA:U6:96Ac. Detailed descriptions of these vectors are available at the CRISPR Fly Design site.

The TRiP encourages the community to nominate genes for TRiP-OE and TRiP-KO production via the TRiP sgRNA Database. If use of any of the TRiP-CRISPR stocks results in data used in a publication, please cite the TRiP in your publication.

** If indicated, this insertion targets more than one gene. To see the other targets, either search this page with the stock number or click on the stock number to read the stock report.

  Assoc. Gene Multiple Targets** Vector Symbol Chr. Stk #
1 Acon pCFD4 P{TOE.GS00476}attP40 2 68079
2 Acox57D-d pCFD4 P{TOE.GS00477}attP40 2 68080
3 Acox57D-p pCFD4 P{TOE.GS00478}attP40 2 68081
4 Actbeta pCFD4 P{TOE.GS00028}attP40 2 67641
5 AdenoK pCFD4 P{TOE.GS00750}attP40 2 76109
6 Ady43A pCFD4 P{TOE.GS00479}attP40 2 68082
7 Alas pCFD4 P{TOE.GS00480}attP40 2 68083
8 Aldh pCFD4 P{TOE.GS00481}attP40 2 68084
9 alpha-Man-Ib multiple targets pCFD4 P{TOE.GS00397}attP40 2 67602
10 aos pCFD4 P{TOE.GS00057}attP40 2 67531
11 ApepP pCFD4 P{TOE.GS00472}attP40 2 76085
12 apt pCFD4 P{TKO.GS00740}attP40 2 76088
13 ATPsynE pCFD3 P{TKO.GS00922}attP40 2 76099
14 ATPsynepsilonL pCFD3 P{TKO.GS00432}attP40 2 68048
15 ATPsynepsilonL pCFD4 P{TOE.GS00352}attP40 2 67595
16 babo pCFD4 P{TOE.GS00401}attP40 2 76081
17 bc10 pCFD3 P{TKO.GS00916}attP40 2 76093
18 bc10 pCFD4 P{TOE.GS00359}attP40 2 67596
19 bgm pCFD4 P{TOE.GS00482}attP40 2 68085
20 bnl pCFD4 P{TOE.GS00107}attP40 2 67542
21 cathD pCFD4 P{TOE.GS00483}attP40 2 68086
22 CG10924 pCFD4 P{TOE.GS00484}attP40 2 68087
23 CG11426 pCFD4 P{TOE.GS00485}attP40 2 68088
24 CG11438 pCFD4 P{TOE.GS00486}attP40 2 68089
25 CG11876 multiple targets pCFD4 P{TOE.GS00397}attP40 2 67602
26 CG11897 pCFD4 P{TOE.GS00540}attP40 2 68143
27 CG11897 pCFD4 P{TOE.GS00541}attP40 2 68144
28 CG12355 pCFD3 P{TKO.GS00921}attP40 2 76098
29 CG12384 pCFD3 P{TKO.GS00437}attP40 2 68052
30 CG12384 pCFD4 P{TOE.GS00293}attP40 2 67577
31 CG12746 pCFD4 P{TOE.GS00487}attP40 2 68090
32 CG1315 pCFD4 P{TOE.GS00488}attP40 2 68091
33 CG13252 pCFD4 P{TOE.GS00195}attP40 2 67561
34 CG14483 pCFD4 P{TOE.GS00267}attP40 2 68014
35 CG1461 pCFD4 P{TOE.GS00489}attP40 2 68092
36 CG14812 pCFD3 P{TKO.GS00925}attP40 2 76102
37 CG14812 pCFD4 P{TOE.GS00336}attP40 2 67590
38 CG14818 pCFD4 P{TOE.GS00288}attP40 2 67576
39 CG14933 pCFD4 P{TOE.GS00298}attP40 2 68023
40 CG14967 pCFD4 P{TOE.GS00185}attP40 2 67557
41 CG1504 pCFD4 P{TOE.GS00075}attP40 2 67534
42 CG15040 pCFD4 P{TOE.GS00086}attP40 2 76075
43 CG15450 pCFD4 P{TOE.GS00490}attP40 2 68093
44 CG15456 pCFD4 P{TOE.GS00265}attP40 2 67566
45 CG15458 pCFD3 P{TKO.GS00917}attP40 2 76094
46 CG15531 pCFD4 P{TOE.GS00491}attP40 2 68094
47 CG15635 pCFD4 P{TOE.GS00015}attP40 2 67518
48 CG15744 pCFD4 P{TOE.GS00072}attP40 2 76078
49 CG15829 pCFD4 P{TOE.GS00282}attP40 2 67573
50 CG16713 pCFD4 P{TOE.GS00270}attP40 2 68015
51 CG17027 pCFD4 P{TOE.GS00492}attP40 2 68095
52 CG17028 pCFD4 P{TOE.GS00493}attP40 2 68096
53 CG17239 pCFD4 P{TOE.GS00153}attP40 2 76076
54 CG17283 pCFD4 P{TOE.GS00494}attP40 2 68097
55 CG17544 pCFD4 P{TOE.GS00495}attP40 2 68098
56 CG17691 pCFD4 P{TOE.GS00496}attP40 2 68099
57 CG17734 pCFD3 P{TKO.GS00918}attP40 2 76095
58 CG17734 pCFD4 P{TOE.GS00261}attP40 2 68012
59 CG17807 pCFD3 P{TKO.GS00412}attP40 2 67605
60 CG18012 pCFD4 P{TOE.GS00346}attP40 2 67593
61 CG18508 pCFD4 P{TOE.GS00317}attP40 2 68026
62 CG18563 pCFD4 P{TOE.GS00082}attP40 2 67536
63 CG2137 pCFD4 P{TOE.GS00741}attP40 2 68145
64 CG2201 pCFD4 P{TOE.GS00435}attP40 2 68051
65 CG2816 pCFD4 P{TOE.GS00285}attP40 2 68019
66 CG30103 pCFD4 P{TOE.GS00497}attP40 2 68100
67 CG30120 multiple targets pCFD4 P{TOE.GS00392}attP40 2 67599
68 CG31075 pCFD4 P{TOE.GS00498}attP40 2 68101
69 CG31468 pCFD4 P{TOE.GS00326}attP40 2 68029
70 CG31473 pCFD4 P{TOE.GS00499}attP40 2 68102
71 CG31869 pCFD4 P{TOE.GS00133}attP40 2 76080
72 CG32069 pCFD4 P{TOE.GS00910}attP40 2 76112
73 CG3209 pCFD4 P{TOE.GS00500}attP40 2 68103
74 CG32448 pCFD4 P{TOE.GS00334}attP40 2 67588
75 CG32698 pCFD4 P{TOE.GS00093}attP40 2 67540
76 CG32736 pCFD4 P{TOE.GS00272}attP40 2 67568
77 CG33096 pCFD4 P{TOE.GS00002}attP40 2 67517
78 CG34172 pCFD4 P{TOE.GS00335}attP40 2 67589
79 CG34228 pCFD4 P{TOE.GS00318}attP40 2 67584
80 CG34229 pCFD4 P{TOE.GS00353}attP40 2 68032
81 CG34263 pCFD4 P{TOE.GS00275}attP40 2 67570
82 CG34276 pCFD4 P{TOE.GS00273}attP40 2 68016
83 CG3513 pCFD4 P{TOE.GS00911}attP40 2 76113
84 CG3566 pCFD4 P{TOE.GS00356}attP40 2 68033
85 CG3809 pCFD4 P{TOE.GS00503}attP40 2 68106
86 CG40228 pCFD4 P{TOE.GS00295}attP40 2 68022
87 CG40472 pCFD4 P{TOE.GS00296}attP40 2 67578
88 CG40485 pCFD4 P{TOE.GS00156}attP40 2 76077
89 CG4101 pCFD3 P{TKO.GS00455}attP40 2 68064
90 CG42259 pCFD3 P{TKO.GS00919}attP40 2 76096
91 CG42259 pCFD4 P{TOE.GS00280}attP40 2 68018
92 CG42308 pCFD4 P{TOE.GS00314}attP40 2 67583
93 CG42375 pCFD4 P{TOE.GS00271}attP40 2 67567
94 CG42381 pCFD3 P{TKO.GS00920}attP40 2 76097
95 CG42456 pCFD4 P{TOE.GS00284}attP40 2 67575
96 CG42460 pCFD4 P{TOE.GS00283}attP40 2 67574
97 CG42464 pCFD4 P{TOE.GS00278}attP40 2 67571
98 CG42486 pCFD4 P{TOE.GS00289}attP40 2 68020
99 CG42496 pCFD4 P{TOE.GS00266}attP40 2 68013
100 CG42497 pCFD3 P{TKO.GS00915}attP40 2 76092
101 CG42538 pCFD3 P{TKO.GS00439}attP40 2 68054
102 CG42566 pCFD4 P{TOE.GS00333}attP40 2 67587
103 CG42716 pCFD3 P{TKO.GS00924}attP40 2 76101
104 CG42717 pCFD4 P{TOE.GS00304}attP40 2 68025
105 CG42787 pCFD4 P{TOE.GS00308}attP40 2 67581
106 CG4302 pCFD4 P{TOE.GS00504}attP40 2 68107
107 CG43069 pCFD4 P{TOE.GS00381}attP40 2 67598
108 CG43229 pCFD4 P{TOE.GS00372}attP40 2 67597
109 CG43324 pCFD4 P{TOE.GS00305}attP40 2 67580
110 CG43350 pCFD4 P{TOE.GS00347}attP40 2 67594
111 CG44194 pCFD4 P{TOE.GS00301}attP40 2 68024
112 CG44296 pCFD4 P{TOE.GS00321}attP40 2 68027
113 CG45011 pCFD4 P{TOE.GS00279}attP40 2 68017
114 CG4650 pCFD4 P{TOE.GS00350}attP40 2 68031
115 CG4660 pCFD4 P{TOE.GS00147}attP40 2 67549
116 CG4998 pCFD4 P{TOE.GS00154}attP40 2 67552
117 CG5009 pCFD4 P{TOE.GS00506}attP40 2 68109
118 CG5189 multiple targets pCFD4 P{TOE.GS00392}attP40 2 67599
119 CG5214 pCFD4 P{TOE.GS00507}attP40 2 68110
120 CG5493 pCFD4 P{TOE.GS00443}attP40 2 68058
121 CG5828 pCFD4 P{TOE.GS00509}attP40 2 68112
122 CG5966 pCFD4 P{TOE.GS00510}attP40 2 68113
123 CG6066 pCFD4 P{TOE.GS00400}attP40 2 67603
124 CG6115 pCFD4 P{TOE.GS00337}attP40 2 67591
125 CG6225 pCFD4 P{TOE.GS00440}attP40 2 68055
126 CG6225 pCFD4 P{TOE.GS00454}attP40 2 76086
127 CG6244 pCFD4 P{TOE.GS00291}attP40 2 68021
128 CG6475 pCFD4 P{TOE.GS00511}attP40 2 68114
129 CG6508 pCFD4 P{TOE.GS00512}attP40 2 68115
130 CG6610 pCFD3 P{TKO.GS01025}attP40 2 76091
131 CG6770 pCFD4 P{TOE.GS00361}attP40 2 68034
132 CG7140 pCFD4 P{TOE.GS00461}attP40 2 68070
133 CG7296 pCFD4 P{TOE.GS00188}attP40 2 67559
134 CG7358 pCFD3 P{TKO.GS00449}attP40 2 68063
135 CG7637 pCFD4 P{TOE.GS00274}attP40 2 67569
136 CG7768 pCFD4 P{TOE.GS00513}attP40 2 68116
137 CG8036 pCFD4 P{TOE.GS00514}attP40 2 68117
138 CG8199 pCFD4 P{TOE.GS00515}attP40 2 68118
139 CG8311 pCFD4 P{TOE.GS00393}attP40 2 68037
140 CG8343 pCFD4 P{TOE.GS00121}attP40 2 76079
141 CG8379 multiple targets pCFD4 P{TOE.GS00395}attP40 2 67600
142 CG8498 pCFD4 P{TOE.GS00404}attP40 2 67604
143 CG8629 pCFD4 P{TOE.GS00325}attP40 2 68028
144 CG8630 pCFD4 P{TOE.GS00516}attP40 2 68119
145 CG9065 pCFD4 P{TOE.GS00327}attP40 2 67585
146 CG9265 pCFD4 P{TOE.GS00517}attP40 2 68120
147 CG9527 pCFD4 P{TOE.GS00441}attP40 2 68056
148 CG9581 pCFD4 P{TOE.GS00913}attP40 2 76083
149 CG9669 pCFD4 P{TOE.GS00281}attP40 2 67572
150 CG9743 pCFD4 P{TOE.GS00744}attP40 2 68148
151 ChAT pCFD4 P{TOE.GS00463}attP40 2 68072
152 chico pCFD4 P{TOE.GS00909}attP40 2 76114
153 cN-IIIB pCFD4 P{TOE.GS00502}attP40 2 68105
154 Coprox pCFD4 P{TOE.GS00521}attP40 2 68124
155 COX6AL pCFD4 P{TOE.GS00263}attP40 2 67565
156 ct pCFD4 P{TOE.GS00041}attP40 2 67524
157 Cyp1 pCFD4 P{TOE.GS00522}attP40 2 68125
158 Cyp18a1 pCFD4 P{TOE.GS00742}attP40 2 68146
159 Cyp9b1 pCFD4 P{TOE.GS00523}attP40 2 68126
160 Cyp9f2 pCFD4 P{TOE.GS00442}attP40 2 68057
161 daw pCFD4 P{TOE.GS00051}attP40 2 67529
162 Dhc36C multiple targets pCFD4 P{TOE.GS00396}attP40 2 67601
163 dnk pCFD4 P{TOE.GS00524}attP40 2 68127
164 dob pCFD4 P{TOE.GS00908}attP40 2 76110
165 dpp pCFD4 P{TOE.GS00163}attP40 2 67554
166 Dtg pCFD4 P{TOE.GS00037}attP40 2 76074
167 egr pCFD4 P{TOE.GS00111}attP40 2 67543
168 Eip55E pCFD4 P{TOE.GS00525}attP40 2 68128
169 elav pCFD4 P{TOE.GS00140}attP40 2 76073
170 fbl pCFD4 P{TOE.GS00526}attP40 2 68129
171 fbp pCFD4 P{TOE.GS00527}attP40 2 68130
172 FK506-bp2 pCFD4 P{TOE.GS00528}attP40 2 68131
173 FKBP59 pCFD4 P{TOE.GS00529}attP40 2 68132
174 gbb pCFD4 P{TOE.GS00073}attP40 2 67533
175 Gclc pCFD3 P{TKO.GS00426}attP40 2 68045
176 Gclc pCFD4 P{TOE.GS00538}attP40 2 68141
177 Gclc pCFD4 P{TOE.GS00539}attP40 2 68142
178 gig pCFD4 P{TOE.GS00753}attP40 2 76116
179 GluRIIA pCFD3 P{TKO.GS00444}attP40 2 68059
180 GluRIIA pCFD4 P{TOE.GS00474}attP40 2 68078
181 GluRIIA pCFD4 P{TOE.GS00927}attP40 2 76107
182 GlyP pCFD4 P{TOE.GS00530}attP40 2 68133
183 Got2 pCFD4 P{TOE.GS01021}attP40 2 76103
184 Gpdh pCFD4 P{TOE.GS00752}attP40 2 76117
185 Gpo-1 pCFD4 P{TOE.GS00531}attP40 2 68134
186 grk pCFD4 P{TOE.GS00045}attP40 2 67525
187 Gs2 pCFD4 P{TOE.GS00532}attP40 2 68135
188 GstE4 pCFD4 P{TOE.GS00533}attP40 2 68136
189 GstE5 pCFD4 P{TOE.GS00534}attP40 2 68137
190 GstE9 pCFD4 P{TOE.GS00464}attP40 2 68073
191 GstT2 pCFD4 P{TOE.GS00743}attP40 2 68147
192 GstT3 pCFD4 P{TOE.GS00465}attP40 2 68074
193 GstZ1 pCFD4 P{TOE.GS00466}attP40 2 68075
194 GstZ2 pCFD4 P{TOE.GS00467}attP40 2 68076
195 Hex-t2 pCFD4 P{TOE.GS00749}attP40 2 76108
196 hh pCFD4 P{TOE.GS00191}attP40 2 67560
197 hll pCFD4 P{TOE.GS00505}attP40 2 68108
198 Hn pCFD4 P{TOE.GS00468}attP40 2 68077
199 HP1Lcsd pCFD4 P{TOE.GS00330}attP40 2 67586
200 IA-2 pCFD4 P{TOE.GS00251}attP40 2 68008
201 Irp-1A pCFD4 P{TOE.GS00420}attP40 2 68039
202 IscU multiple targets pCFD4 P{TOE.GS00395}attP40 2 67600
203 jeb pCFD4 P{TOE.GS00159}attP40 2 67553
204 Krn pCFD4 P{TOE.GS00186}attP40 2 67558
205 l(1)G0334 pCFD4 P{TOE.GS00446}attP40 2 68061
206 l(3)02640 pCFD4 P{TOE.GS00421}attP40 2 68040
207 lace pCFD4 P{TOE.GS00422}attP40 2 68041
208 laza pCFD4 P{TOE.GS00429}attP40 2 68046
209 LManI pCFD4 P{TOE.GS00508}attP40 2 68111
210 LManIII pCFD4 P{TOE.GS00518}attP40 2 68121
211 LManIV pCFD4 P{TOE.GS00519}attP40 2 68122
212 LManV pCFD4 P{TOE.GS00520}attP40 2 68123
213 Lon pCFD4 P{TOE.GS00471}attP40 2 76084
214 LPCAT pCFD4 P{TOE.GS00501}attP40 2 68104
215 mav pCFD4 P{TOE.GS00018}attP40 2 67520
216 mEFTs multiple targets pCFD4 P{TOE.GS00396}attP40 2 67601
217 Membrin multiple targets pCFD4 P{TOE.GS00389}attP40 2 68036
218 Mgstl pCFD4 P{TOE.GS00430}attP40 2 68047
219 muc pCFD4 P{TOE.GS00462}attP40 2 68071
220 myo pCFD4 P{TOE.GS00047}attP40 2 67526
221 Nach pCFD3 P{TKO.GS00249}attP40 2 68006
222 Nach pCFD4 P{TOE.GS00257}attP40 2 67562
223 nct pCFD3 P{TKO.GS00436}attP40 2 76090
224 ND-39 pCFD4 P{TOE.GS00387}attP40 2 68035
225 ND-AGGG pCFD4 P{TOE.GS00345}attP40 2 67592
226 ND-MLRQ pCFD4 P{TOE.GS00297}attP40 2 67579
227 Nmnat pCFD4 P{TOE.GS00423}attP40 2 68042
228 peb pCFD4 P{TOE.GS00052}attP40 2 67530
229 ppk16 pCFD3 P{TKO.GS00252}attP40 2 68009
230 ppk16 pCFD4 P{TOE.GS00259}attP40 2 67564
231 ppk28 pCFD3 P{TKO.GS00410}attP40 2 68038
232 ppk6 pCFD3 P{TKO.GS00254}attP40 2 68010
233 ppk6 pCFD4 P{TOE.GS00258}attP40 2 67563
234 pps pCFD3 P{TKO.GS00445}attP40 2 68060
235 pr pCFD4 P{TOE.GS00456}attP40 2 68065
236 Psn pCFD3 P{TKO.GS00748}attP40 2 76089
237 Pu pCFD4 P{TOE.GS00433}attP40 2 68049
238 Pvf1 pCFD4 P{TOE.GS00032}attP40 2 67522
239 Pvf2 pCFD4 P{TOE.GS00162}attP40 2 67634
240 Pvf3 pCFD4 P{TOE.GS00022}attP40 2 67521
241 PyK pCFD4 P{TOE.GS00751}attP40 2 76111
242 pyr pCFD4 P{TOE.GS00085}attP40 2 67537
243 QUAS pCFD4 P{GS00089}attP40 2 67539
244 r pCFD4 P{TOE.GS00457}attP40 2 68066
245 RNASEK pCFD3 P{TKO.GS00438}attP40 2 68053
246 ry pCFD4 P{TOE.GS00458}attP40 2 68067
247 Sam-S pCFD4 P{TOE.GS00434}attP40 2 68050
248 Sbf pCFD4 P{TOE.GS01026}attP40 2 76115
249 Scsalpha pCFD4 P{TOE.GS01022}attP40 2 76104
250 scw pCFD4 P{TOE.GS00080}attP40 2 67535
251 skap pCFD4 P{TOE.GS00535}attP40 2 68138
252 SLIRP2 pCFD4 P{TOE.GS00332}attP40 2 68030
253 snf pCFD3 P{TKO.GS00425}attP40 2 68044
254 spo pCFD4 P{TOE.GS00907}attP40 2 76105
255 Spt-I pCFD4 P{TOE.GS00537}attP40 2 68140
256 spz pCFD4 P{TOE.GS00152}attP40 2 67551
257 spz3 pCFD4 P{TOE.GS00008}attP40 2 68005
258 spz4 pCFD4 P{TOE.GS00016}attP40 2 67519
259 spz5 pCFD4 P{TOE.GS00130}attP40 2 67547
260 spz6 pCFD4 P{TOE.GS00088}attP40 2 67538
261 ths pCFD4 P{TOE.GS00134}attP40 2 67635
262 Trh pCFD4 P{TOE.GS00459}attP40 2 68068
263 trk pCFD4 P{TOE.GS00062}attP40 2 67532
264 Uev1A multiple targets pCFD4 P{TOE.GS00389}attP40 2 68036
265 Ufm1 pCFD3 P{TKO.GS00923}attP40 2 76100
266 Ugt86De pCFD4 P{TOE.GS00460}attP40 2 68069
267 upd1 pCFD4 P{TOE.GS00169}attP40 2 67555
268 upd2 pCFD4 P{TOE.GS00171}attP40 2 67556
269 upd3 pCFD4 P{TOE.GS00129}attP40 2 67546
270 v pCFD4 P{TOE.GS00926}attP40 2 76106
271 veil pCFD4 P{TOE.GS00424}attP40 2 68043
272 vn pCFD4 P{TOE.GS00144}attP40 2 67548
273 Vti1b pCFD4 P{TOE.GS00312}attP40 2 67582
274 wg pCFD4 P{TOE.GS00125}attP40 2 67545
275 whd pCFD4 P{TOE.GS00536}attP40 2 68139
276 Wnt10 pCFD4 P{TOE.GS00034}attP40 2 67523
277 Wnt2 pCFD4 P{TOE.GS00120}attP40 2 67544
278 Wnt4 pCFD4 P{TOE.GS00048}attP40 2 67527
279 Wnt5 pCFD4 P{TOE.GS00148}attP40 2 67550
280 Wnt6 pCFD4 P{TOE.GS00049}attP40 2 67528
281 wntD pCFD4 P{TOE.GS00104}attP40 2 67541
282 zfh2 pCFD4 P{TKO.GS00475}attP40 2 76087
283 ZnT33D pCFD4 P{TOE.GS00255}attP40 2 68011
284 ZnT35C pCFD4 P{TOE.GS00250}attP40 2 68007
285 ZnT63C pCFD3 P{TKO.GS00447}attP40 2 68062
286 ZnT63C pCFD4 P{TOE.GS01024}attP40 2 76082