Stocks carrying p65 activation domain hemidrivers are listed here. The vast majority of these stocks were donated by the Janelia Research Campus and were generated in the lab of Gerald Rubin at Janelia or in the lab of Barry Dickson at the Institute of Molecular Pathology in Vienna. The methods and characterization of these lines is described in Dionne et al., 2017 and Tirian and Dickson, 2017.
Predicting the expression pattern of Janelia split-GAL4 hemidrivers
Each regulatory fragment for a given split-GAL4 hemidriver has also been used to drive expression of GAL4, and the expression pattern of these full GAL4 constructs was described by Janelia Research Campus, Barry Dickson's group at the Institute of Molecular Pathology, or Andrew Stark's group at the Institute of Molecular Pathology. Based on data from Dionne et al., 2017 and personal communication with Janelia, the expression of a full GAL4 is highly predictive of the expression of a split-GAL4 hemidriver carrying the same regulatory fragment for the DBD hemidrivers inserted in attP2, while the AD hemidrivers inserted in attP40 may differ (see the Janelia lexA page for important information on potential expression differences between insertions carrying the same sequence fragment, but inserted in different attP sites). We provide links to expression data for each full GAL4 driven by the regulatory fragment found in a corresponding split-GAL4 hemidriver.
Control stocks for the Janelia GAL4 DBD and AD split lines:
This control construct carries the DNA binding domain of GAL4 in the same vector used to make the Janelia split GAL4 collection but with no regulatory fragment present. Also known as the 'GAL4-DBD.empty' control, BPZpGDBD or pBPZpGAL4DBDUw (attP2).
This control construct carries the p65 activation domain in the same vector used to make the Janelia split GAL4 collection but with no regulatory fragment present. Also known as the 'p65-AD.empty' control, BPp65ADZp or pBPp65ADZpUw (attP40).