CoinFLP stocks
Updated June 29, 2017
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P{CoinFLP-GAL4} carries overlapping FRT and FRT3 cassettes. FLP recombinase can induce recombination between FRT or FRT3 pairs, but FRT and FRT3 are incompatible. When FLP is expressed, one cassette or the other is removed stochastically. Removal of the FRT cassette allows GAL4 expression.

Figure showing P{CoinFLP-GAL4} use in generating clones from Bosch et al. (2015) CoinFLP: A system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila, Development 142: 597-606

P{CoinFLP-LexA.GAL4} is a similar construct where LexA is expressed upon removal of the FRT cassette and GAL4 is expressed upon removal of the FRT3 cassette.

Figure showing P{CoinFLP-LexA.GAL4} use in generating clones from Bosch et al. (2015) CoinFLP: A system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila, Development 142: 597-606

If P{CoinFLP-LexA.GAL4} is combined with the split GFP constructs P{UAS-CD4-spGFP1-10} and P{lexAop-CD4-spGFP11}, the interface between the two kinds of clones will be marked with GFP.

Figure showing use of P{CoinFLP-LexA.GAL4} with the split GFP system to show the interface between clones from Bosch et al. (2015) CoinFLP: A system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila, Development 142: 597-606

These constructs and methods will be described in Bosch et al. (2015) "CoinFLP: A system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila" Development 142: 597-606.

Stocks originating in this work are shown below.

Go to the main FLP/FRT and other recombinases page.
Stock # Genotype
Chromosomes
59269 w[*] P{y[+t7.7] w[+mC]=CoinFLP-GAL4}attP3
1
58750 w[*]; P{y[+t7.7] w[+mC]=CoinFLP-GAL4}attP40/CyO; Dr[1]/TM6C, Sb[1]
1;2;3
58751 w[*]; P{y[+t7.7] w[+mC]=CoinFLP-GAL4}attP40 P{w[+mC]=UAS-2xEGFP}AH2
1;2
58752 w[*]; P{y[+t7.7] w[+mC]=CoinFLP-GAL4}attP40; P{y[+t7.7] v[+t1.8]=TRiP.HMS00017}attP2
1;2;3
59268 w[*]; wg[Sp-1]/CyO; P{y[+t7.7] w[+mC]=CoinFLP-GAL4}attP2/TM6C, Sb[1] Tb[1]
1;2;3
59271 w[*] P{y[+t7.7] w[+mC]=CoinFLP-LexA.GAL4}attP3
1
58753 w[*]; P{y[+t7.7] w[+mC]=CoinFLP-LexA.GAL4}attP40/CyO; Dr[1]/TM6C, Sb[1]
1;2;3
58754 y[1] w[*] P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP18 P{y[+t7.7] w[+mC]=13XLexAop2-mCD8::GFP}su(Hw)attP8; P{y[+t7.7] w[+mC]=CoinFLP-LexA.GAL4}attP40
1;2
58755 w[*]; P{y[+t7.7] w[+mC]=CoinFLP-LexA.GAL4}attP40 P{w[+mC]=lexAop-rCD2.RFP}2; P{w[+mC]=UAS-CD4-spGFP1-10}3, P{w[+mC]=lexAop-CD4-spGFP11}3/TM6C, Sb[1]
1;2;3
59270 w[*]; wg[Sp-1]/CyO; P{y[+t7.7] w[+mC]=CoinFLP-LexA.GAL4}attP2/TM6C, Sb[1] Tb[1]
1;2;3