All Janelia Farm GAL4 stocks (a.k.a. "GMR_Brain_exp_1" or "Rubin GAL4" lines) currently available at Bloomington are listed here. These lines express GAL4 under the control of defined sequence fragments from either flanking non-coding or intronic regions of associated genes. In general, these drivers express GAL4 in more restricted patterns than observed with standard enhancer traps. All the constructs were inserted by site-specific recombination into the attP2 site at 68A4 on 3L.
We anticipate adding approximately 400 stocks a month to the list until mid-2012 (for a total of ~7000 stocks).
The fragment reports linked to below contain a GBrowse view of the fragment location. The FlyBase report on the collection can be found here. Files containing construct information, including primers and sequence coordinates for the fragments in the constructs, can be downloaded as an Excel file or a csv file (please note these files contain all 7128 lines - including those not yet available at Bloomington).
This collection was constructed by the methods described in Pfeiffer et. al., 2008.
Please cite those who generated and analyzed these materials when publishing your own work with these and other Stock Center stocks.
IMPORTANT! Please read cautionary information on the Janelia GAL4 stocks here.
A list of tested stocks, their test results and their status at the BDSC can be downloaded as an Excel file or a csv file. Sequence-verified stocks are also indicated below.