All Janelia Farm GAL4 stocks (a.k.a. "GMR_Brain_exp_1" or "Rubin GAL4" lines) currently available at Bloomington are listed here (we anticipate that all available stocks will be in distribution by mid-October). These lines express GAL4 under the control of defined sequence fragments from either flanking non-coding or intronic regions of associated genes. In general, these drivers express GAL4 in more restricted patterns than observed with standard enhancer traps.
Expression Data - Data on the expression patterns generated by these GAL4 lines in the adult brain and ventral nerve cord, in third instar imaginal discs, and in the embryonic CNS can be found at Janelia's FlyLight site. Links to the data for individual lines are provided in the "Expression data" column below. The data presented at FlyLight are the work of the Janelia Farm FlyLight Project Team and the laboratories of Gerald M. Rubin, Richard S. Mann and Christopher Q. Doe.
Fragment information - The fragment reports linked to below contain a GBrowse view of the fragment location. The FlyBase report on the collection can be found here. Files containing construct information, including primers and sequence coordinates for the fragments in the constructs, can be downloaded as an Excel file or a csv file (please note these files contain lines not yet available).
Stock/Insertion information - All the constructs were inserted by site-specific recombination into the attP2 site at 68A4 on 3L. The line used by Janelia Farms for balancing and stocking these strains is available as BDSC Stk#36305 w; Dr e/TM3, Sb
Download a stocklist - A list of Janelia stocks available from the BDSC with their stock numbers can be downloaded as an Excel file or a csv file. Stock numbers can be cut and pasted from these files into our ordering page.
IMPORTANT! Please read cautionary information on the Janelia GAL4 stocks here.