Stocks for isolating RNA from specific cells
Updated January 18, 2013
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Translating Ribosomal Affinity Purification (TRAP)

Thomas et al. (2012) created stocks for expressing a GFP-tagged ribosomal protein under UAS control. Ribosomes from GAL4-expressing cells can be recovered by immunoprecipitation with an anti-GFP antibody and actively translated mRNAs isolated.

 
Stock #
Genotype
1
w[*]; P{UAS-GFP-RpL10Ab}BF2b
2
P{UAS-GFP-RpL10Ab}BF24, w[*]; l(2)*[*]/CyO
3
w[*]; P{UAS-GFP-RpL10Ab}BF27

TU-tagging

Miller et al. (2009) generated stocks to express uracil phosphoribosyltransferase (UPRT) under UAS control. In flies fed 4-thiouracil, 4-thiouracil is modified in GAL4-expressing cells and incorporated into mRNA. Thio-labeled mRNA can be selectively recovered by affinity purification.

 
Stock #
Genotype
1 w[*]; P{UAS-HA-UPRT}2.1; Dr[1]/TM3, Sb[1]
2 w[*]; wg[Sp-1]/CyO; P{UAS-UPRT.M}3.1
3 w[*]; wg[Sp-1]/CyO; P{UAS-HA-UPRT}3.2