The TIE-DYE system for marking mitotic clones
Updated September 2, 2013
Stock Center logo

The TIE-DYE system uses heat shock-driven FLP recombinase to excise transcriptional stop cassettes stochastically from three constructs to generate clones expressing single markers or combinations of markers. Any additional UAS constructs will be expressed in RFP-marked clones.

TIE-DYE illustration originating in Worley et al. (2013), TIE-DYE: a combinatorial marking system to visualize and genetically manipulate clones during development in Drosophila melanogaster. Development 140: 3275-3284

The method was described in Worley et al. (2013). TIE-DYE: a combinatorial marking system to visualize and genetically manipulate clones during development in Drosophila melanogaster.


  Stock # Genotype
1 51308 w[*]; P{Act5C(FRT.polyA)lacZ.nls1}2, P{Ubi-p63E(FRT.STOP)Stinger}9F6/CyO; P{GAL4-Act5C(FRT.CD2).P}S, P{UAS-His-RFP}3/TM6B, Tb[1]