Stocks for generating cell lines
Updated December 8, 2015
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Combining P{UAS-Ras85D.V12} with a GAL4 driver will result in oncogenic transformation of cells from the expression of constitutively active Ras85D. These overproliferating cells can be used to establish cell lines in tissue culture as shown in the figure below.

Figure showing the establishment of oncogenic cell lines from fly tissue expressing constitutively active Ras85D from Manivannan et al. (2015) Targeted Integration of Single-Copy Transgenes in Drosophila melanogaster Tissue-Culture Cells Using Recombination Mediated Cassette Exchange. Genetics.

A transgene can be introduced into attP target sites in a progenitor fly line by RMCE so that it will be present once transformed cell lines are established.

The method was described in Manivannan et al. (2015). Targeted Integration of Single-Copy Transgenes in Drosophila melanogaster Tissue-Culture Cells Using Recombination Mediated Cassette Exchange. Genetics 201: 1319-1328.

  Stock # Genotype
Chromosomes
1 64195 w[*]; P{w[+mC]=UAS-Ras85D.V12}TL1 1;3
2 64196 w[*]; P{w[+mC]=UAS-Ras85D.V12}2 1;2
3 64197 w[*]; P{w[+mC]=UAS-Ras85D.V12}TL1, P{w[+mC]=attP.w[+].attP}JB89B 1;3
4 3954 y[1] w[*]; P{w[+mC]=Act5C-GAL4}17bFO1/TM6B, Tb[1] 1;3