deGradFP stocks for protein inactivation
Updated December 31, 2014
Stock Center logo

deGradFP (degrade Green Fluorescent Protein) is a method for inactivating GFP-tagged proteins. A single-domain antibody fragment against GFP (vhhGFP4) is fused to the F-box domain from the slmb protein N-terminus (Nslmb) and expressed under UAS control. The fusion protein binds GFP and directs it to the proteasome for degradation. If gene function is provided only by a GFP fusion construct, expression of Nslmb-vhhGFP4 protein from P{UAS-Nslmb-vhhGFP4} results in loss-of-function phenotypes in GAL4-expressing cells.

Reference: Caussinus et al. (2011). Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Nat. Struct. Mol. Biol. 19: 117--121.

  Stock # Genotype
Chromosomes
1 38422 y[1] w[*]; P{w[+mC]=UAS-Nslmb-vhhGFP4}2
1;2
2 58740 y[1] w[*]; M{w[+mC]=UASp-Nslmb.vhhGFP4}ZH-51D
1;2
3 38421 w[*]; P{w[+mC]=UAS-Nslmb-vhhGFP4}3
1;3
4 58739 w[*]; M{w[+mC]=UASp-Nslmb.vhhGFP4}ZH-86Fb2
1;3
5 38417 w[*]; P{w[+mC]=nullo-GAL4.G}5.20/CyO; P{w[+mC]=UAS-Nslmb-vhhGFP4}3
1;2;3
6 38418 w[*]; P{w[+mW.hs]=GawB}nubbin-AC-62/CyO; P{w[+mC]=UAS-Nslmb-vhhGFP4}3/TM3, Sb[1] Ser[1]
1;2;3
7 38419 w[*]; P{w[+mW.hs]=GawB}332.3; P{w[+mC]=UAS-Nslmb-vhhGFP4}3
1;2;3
8 38420 w[*]; P{w[+mW.hs]=en2.4-GAL4}e16E, P{w[+mC]=UAS-mCherry.NLS}2; P{w[+mC]=UAS-Nslmb-vhhGFP4}3/TM3, Sb[1]
1;2;3