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Defined FLP variants
Variant FLP recombinase proteins with different activity levels have been isolated. Most fly constructs appear to express one of two FLP variants (FLPD5 or FLPG5, see below), however, other variants also exist. We do not know the FLP allele present in most Bloomington stocks. The table below shows stocks where we do know the FLP variant expressed.

Known variants:
FLPD5 and FLPG5 - FLP with either glycine (FLPG5) or aspartic acid (FLPD5) at amino acid 5. The FLPD5 version has more than tenfold higher activity than the FLPG5 version according to Nern et al. 2011.

FLPL with aspartic acid at amino acid 5 (D5) and leucine at amino acid 70. It appears to have intermediate activity between the D5 and G5 variants.

FLPL2 with glycine at amino acid position 5 (G5) and leucine at position 70.

FLPD5.fco is a fly codon-optimized FLP with aspartic acid at amino acid 5 (D5). We have no information about its relative efficiency.

FLPo is a mouse codon optimized FLP. We have no information about its relative efficiency.

In the following stocks, some FLP variants are tagged with a PEST sequence to shorten their half-lives. FLP expression can also be driven by different numbers of UAS repeats.

Go to the main FLP/FRT and other recombinases page.
  FLP variant Stk # Chr FLP promoter Genotype
1 FLPD5 55815 1 Hsp70Bb w[1118] P{y[+t7.7] w[+mC]=hs-FLPD5}attP3
2 FLPD5 55814 2 Hsp70Bb w[1118]; P{y[+t7.7] w[+mC]=hs-FLPD5}attP40/CyO
3 FLPD5 55813 3 Hsp70Bb w[1118]; P{y[+t7.7] w[+mC]=hs-FLPD5}attP2
4 FLPD5 55804 3 UAS w[1118]; P{y[+t7.7] w[+mC]=20XUAS-FLPD5.PEST}attP2
5 FLPD5 55805 3 UAS w[1118]; P{y[+t7.7] w[+mC]=20XUAS-FLPD5}attP2
6 FLPD5.fco 55818 1 Hsp70Bb w[1118] P{y[+t7.7] w[+mC]=hs-FLPD5.fco}attP3
7 FLPD5.fco 56749 2 Hsp70Bb w[1118]; P{y[+t7.7] w[+mC]=hs-FLPD5.fco}attP40
8 FLPG5 55765 2 E(spl)m4-BFM w[*]; P{y[+t7.7] w[+mC]=R27G05-FLPG5.PEST}attP40/CyO, P{w[+mC]=GAL4-Kr.C}DC3, P{w[+mC]=UAS-GFP.S65T}DC7; TM2/TM6B, Tb[1]
9 FLPG5 62118 1 Hsp70Bb w[1118] P{y[+t7.7] w[+mC]=hs-FLPG5.PEST}attP3
10 FLPG5 55817 1 Hsp70Bb w[1118] P{y[+t7.7] w[+mC]=hs-FLPG5}attP3
11 FLPG5 58356 2 Hsp70Bb w[1118]; P{y[+t7.7] w[+mC]=hs-FLPG5}attP40
12 FLPG5 55816 3 Hsp70Bb w[1118]; P{y[+t7.7] w[+mC]=hs-FLPG5}attP2/TM3, Sb[1]
13 FLPG5 55768 1 sens w[*] P{y[+t7.7] w[+mC]=sens-FLPG5.C}attP18; wg[Sp-1]/CyO; sens[Ly-1]/TM6B, Tb[1]
14 FLPG5 55807 1 UAS w[1118] P{y[+t7.7] w[+mC]=20XUAS-FLPG5.PEST}attP18
15 FLPG5 55808 1 UAS w[1118] P{y[+t7.7] w[+mC]=3XUAS-FLPG5.PEST}su(Hw)attP8
16 FLPG5 62139 2 UAS w[1118]; P{y[+t7.7] w[+mC]=5xUAS-IVS-FLPG5}attP40
17 FLPG5 55806 2 UAS w[1118]; P{y[+t7.7] w[+mC]=20XUAS-FLPG5.PEST}attP40
18 FLPG5 55809 2 UAS w[1118]; P{y[+t7.7] w[+mC]=3XUAS-FLPG5.PEST}attP40
19 FLPL 55820 2 lexA operator w[1118]; P{y[+t7.7] w[+mC]=8XLexAop2-FLPL}attP40
20 FLPL 55819 3 lexA operator w[1118]; P{y[+t7.7] w[+mC]=8XLexAop2-FLPL}attP2
21 FLPL 62141 2 UAS w[1118]; P{y[+t7.7] w[+mC]=3xUAS-IVS-FLPL}attP40
22 FLPL2 62140 2 UAS w[1118]; P{y[+t7.7] w[+mC]=3xUAS-IVS-FLPL2}attP40
23 FLPo 30126 2 QUAS w[1118]; P{w[+mC]=QUAS-FLPo.P}1
24 FLPo 30127 3 QUAS w[1118]; P{w[+mC]=QUAS-FLPo.P}7
25 FLPo 30008 3 QUAS w[1118]; P{y[+t7.7] w[+mC]=QUAS-DSCP-FLPo.2G}attP2