| Targeted Gene Knock-Out Stocks Updated February 26, 2009 |
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These stocks constitute the Golic lab gene replacement kit for creating targeted knock-outs.
Stock # |
Genotype | |
|---|---|---|
| 1 | w[1118]; P{70FLP}10 | |
| 2 | y[1] w[*]; P{70FLP}11 P{70I-SceI}2B noc[Sco]/CyO, S[2] | |
| 3 | y[1] w[*]; P{70FLP}23 P{70I-SceI}4A/TM6 | |
| 4 | P{hs-I-CreI.R}2A, v[1]; ry[506] | |
| 5 | w[1118]; P{hs-I-CreI.R}1A Sb[1]/TM6 | |
| 6 | y[1] w[*]; P{y-donor}1B Sb[1]/TM6 | |
| 7 | w[1118]; P{w8,I-site}7 |
These stocks are similar to stocks 6934 and 6935, but they allow heat shock killing of unwanted progeny classes in knock-out screens. Their use is described in Huang et al. (2008).
Stock # |
Genotype | |
|---|---|---|
| 1 | y[1] w[*]/Dp(2;Y)G, P{hs-hid}Y; P{70FLP}11 P{70I-SceI}2B noc[Sco]/CyO, P{hs-hid}4 | |
| 2 | y[1] w[*]/Dp(2;Y)G, P{hs-hid}Y; P{70FLP}23 P{70I-SceI}4A/TM3, P{hs-hid}14, Sb[1] |