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Histone mutant stocks
Updated July 11, 2016
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The following stocks contain transgenes carrying three tandem copies of the histone repeat (His1, His2A, His2B, His3 and His4) with modifications made to particular histone genes.

Figure of DNA wrapping around histones in Graves et al. (2016) "Mutations that prevent or mimic persistent post-translational modifications of the histone H3 globular domain cause lethality and growth defects in Drosophila"  Epigenetics & Chromatin 9:9.

  Stk # Genotype
1 65301 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.wt}VK00027 PBac{3xHisGU.wt}VK00033/TM6B, Tb[1]
2 65302 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3K56R}VK00027 PBac{3xHisGU.H3K56R}VK00033
3 65303 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3K56Q}VK00027 PBac{3xHisGU.H3K56Q}VK00033/TM6B, Tb[1]
4 65304 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3K115Q}VK00027 PBac{3xHisGU.H3K115Q}VK00033
5 65305 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3K115Q.H3K122Q}VK00027 PBac{3xHisGU.H3K115Q.H3K122Q}VK00033
6 65306 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3K115R}VK00027 PBac{3xHisGU.H3K115R}VK00033
7 65307 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3K115R.H3K122R}VK00027 PBac{3xHisGU.H3K115R.H3K122R}VK00033
8 65308 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3K122Q}VK00027 PBac{3xHisGU.H3K122Q}VK00033/TM6B, Tb[1]
9 65309 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3K122R}VK00027 PBac{3xHisGU.H3K122R}VK00033/TM6B, Tb[1]
10 65310 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3T80A}VK00027 PBac{3xHisGU.H3T80A}VK00033/TM6B, Tb[1]
11 65311 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3T80E}VK00027 PBac{3xHisGU.H3T80E}VK00033/TM6B, Tb[1]
12 65312 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3T118A}VK00027 PBac{3xHisGU.H3T118A}VK00033
13 65313 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3T118E}VK00027 PBac{3xHisGU.H3T118E}VK00033/TM6B, Tb[1]
14 65314 w[*]; PBac{y[+mDint2] w[+mC]=3xHisGU.H3T118I}VK00027 PBac{3xHisGU.H3T118I}VK00033

To replace normal histones with histones encoded by these transgenes, the transgenes may be crossed into a background where the histone cluster has been deleted by Df(2L)His[C] or Df(2L)BSC104 (stock 8670).

These stocks were described in Graves et al. (2016) "Mutations that prevent or mimic persistent post‑translational modifications of the histone H3 globular domain cause lethality and growth defects in Drosophila" Epigenetics & Chromatin 9:9.