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Lineage restricted expression via patterned recombination

Enhancers often drive gene expression in too many cells to be experimentally useful. To restrict gene expression to a smaller number of cells, one can “filter” expression using a flip-out cassette construct as shown in the example below.

Illustration of lineage-restricted expression of a reporter gene following recombinase-mediated cassette removal from Awasaki et al. (2014), Nature Neuroscience 17:631-637

Stocks useful for filtering gene expression through multiple rounds of recombinase-mediated cassette removal are shown below.

This method was described in Awasaki et al. (2014) "Making Drosophila lineage-restricted drivers via patterned recombination in neuroblasts", Nature Neuroscience 4: 631-637.

  Promoter Stop cassette Gene expressed Stk # Genotype
1 dpn KDRT cre.PEST 56164 w[*] P{y[+t7.7] w[+mC]=dpn(KDRT.stop)cre.PEST}su(Hw)attP8
2 dpn FRT cre.PEST 56165 w[*] P{y[+t7.7] w[+mC]=dpn(FRT.stop)cre.PEST}su(Hw)attP8
3 dpn FRT lexA.p65 56162 w[*] P{y[+t7.7] w[+mC]=dpn(FRT.stop)LexA.p65}su(Hw)attP8
4 nSyb FRT lexA.p65 56163 w[*]; P{y[+t7.7] w[+mC]=nSyb(FRT.stop)LexA.p65}attP2
5 nSyb loxP lexA.p65 56166 w[*]; PBac{y[+mDint2] w[+mC]=nSyb(loxP.stop)LexA.p65}VK00027