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Transcriptional reporters for sensing Ca2+
See below for stocks carrying constructs for transcriptional readout of Ca2+ based on either 1) NFAT or 2) Calmodulin. To browse stocks carrying fluorescent-based Ca2+ sensors see the fluor-based Ca2+ sensor page.

Go to the fluorescent protein home page.
Go to the UAS home page.
 
NFAT - The following stocks carry NFAT, deleted for the C-terminus and fused to lexA and VP16, expressed under the control of UAS. Ca2+ accumulation in activated neurons results in dephosphorylation of NFAT and translocation of the chimera to the nucleus where it can activate a lexAop-driven reporter. For more information see Masuyama et al., 2012.

Note Stk # Genotype
66543 w[*]; P{w[+mC]=UAS-mLexA-VP16-NFAT}H2/TM3, Sb[1]
66542 w[*]; P{w[+mC]=LexAop-CD8-GFP-2A-CD8-GFP}2; P{w[+mC]=UAS-mLexA-VP16-NFAT}H2, P{w[+mC]=lexAop-rCD2-GFP}3/TM6B, Tb[1]
control 66544 w[*]; Pin[1]/CyO; P{w[+mC]=lexAop-rCD2-GFP}3
control 66545 w[*]; P{w[+mC]=LexAop-CD8-GFP-2A-CD8-GFP}2; TM2/TM6B, Tb[1]
 
Calmodulin - TRIC (Transcriptional Reporter of Intracellular Ca2+) is a expression system generated in Gao et al., 2015 designed to report interactions between calmodulin (CaM) and a calmodulin target peptide in the presence of Ca2+. The system is composed of calmodulin fused to the p65 transcriptional activation domain plus one of three calmodulin target peptides (MKII, MKIIK11A or M13) fused to a DNA-binding domain. In the presence of Ca2+, calmodulin binds to its target peptide, reconstituting a transcriptional activator that can then drive expression of a reporter. This system may be particularly useful for monitoring slowly changing neuronal responses that occur over long periods of time.

  target peptide CaM other Stk # Genotype
Stocks carrying a single component
1 nSyb-M13::GAL4DBDo 61662 w[*]; P{y[+t7.7] w[+mC]=nSyb-M13::GAL4DBDo}attP24/CyO; Pri[1]/TM6B, Tb[1]
2 nSyb-MKII::GAL4DBDo 61663 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::GAL4DBDo}attP24/CyO; Pri[1]/TM6B
3 nSyb-MKII::nlsLexADBDo 61665 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::nlsLexADBDo}attP24/CyO; Pri[1]/TM6B
4 UAS-MKII::nlsLexADBDo 61667 w[*]; P{y[+t7.7] w[+mC]=UAS-MKII::nlsLexADBDo}attP40/CyO; Pri[1]/TM6B, Tb[1]
5 UAS-MKII::nlsLexADBDo 61668 w[*]; S[1]/CyO; PBac{y[+mDint2] w[+mC]=UAS-MKII::nlsLexADBDo}VK00005
6 nSyb-MKIIK11A::GAL4DBDo 61664 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKIIK11A::GAL4DBDo}attP24/CyO; Pri[1]/TM6B, Tb[1]
7 nSyb-MKIIK11A::nlsLexADBDo 61666 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKIIK11A::nlsLexADBDo}attP24/CyO; Pri[1]/TM6B, Tb[1]
8 UAS-p65AD::CaM 62829 w[*]; P{y[+t7.7] w[+mC]=UAS-p65AD::CaM}attP24/CyO; Pri[1]/TM6B, Tb[1]
9 UAS-p65AD::CaM 61661 w[*]; S[1]/CyO; M{UAS-p65AD::CaM}ZH-86Fb
10 QUAS-p65AD::CaM 61658 P{w[+mC]=QUAS-p65AD::CaM}1, w[*]; S[1]/CyO; Pri[1]/TM6B, Tb[1]
11 QUAS-p65AD::CaM 62566 w[*]; P{w[+mC]=QUAS-p65AD::CaM}2/CyO; Pri[1]/TM6B, Tb[1]
12 QUAS-p65AD::CaM 61811 w[*]; S[1]/CyO; P{w[+mC]=QUAS-p65AD::CaM}3/TM6B, Tb[1]
13 QUAS(FRT.stop)p65AD::CaM 61659 w[*]; P{w[+mC]=QUAS(FRT.stop)p65AD::CaM}5; Pri[1]/TM6B, Tb[1]
14 QUAS(FRT.stop)p65AD::CaM 61660 w[*]; P{w[+mC]=QUAS(FRT.stop)p65AD::CaM}6
Multi-component stocks with a QF construct
1 nSyb-MKII::GAL4DBDo QUAS-p65AD::CaM UAS-RFP; LexAop2-GFP; nSyb-QF2; tub-QS 61670 w[*] P{y[+] w[+]=10XUAS-IVS-mCD8::RFP}attP18 P{y[+] w[+]=13XLexAop2-mCD8::GFP}su(Hw)attP8; P{y[+] w[+]=nSyb-MKII::GAL4DBDo}attP24, P{w[+]=QUAS-p65AD::CaM}2/CyO; P{y[+] w[+]=nSyb-QF2.P}attP2 P{w[+]=tub-QS.P}9B
2 nSyb-MKII::GAL4DBDo QUAS-p65AD::CaM UAS-GFP; nSyb-QF2; tub-QS 61671 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::GAL4DBDo}attP24, P{w[+mC]=QUAS-p65AD::CaM}2, P{w[+mC]=UAS-mCD8::GFP.L}LL5/CyO; P{y[+t7.7] w[+mC]=nSyb-QF2.P}attP2, P{w[+mC]=tub-QS.P}9B
3 nSyb-MKII::GAL4DBDo QUAS(FRT.stop)p65AD::CaM nSyb-QF2 61674 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::GAL4DBDo}attP24; P{w[+mC]=QUAS(FRT.stop)p65AD::CaM}6, P{y[+t7.7] w[+mC]=nSyb-QF2.P}attP2
4 nSyb-MKII::nlsLexADBDo QUAS-p65AD::CaM nSyb-QF2; tub-QS; LexAop2-GFP 64348 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::nlsLexADBDo}attP24, P{w[+mC]=QUAS-p65AD::CaM}2, P{y[+t7.7] w[+mC]=13XLexAop2-mCD8::GFP}attP40/CyO; P{y[+t7.7] w[+mC]=nSyb-QF2.P}attP2, P{w[+mC]=tub-QS.P}9B
5 nSyb-MKII::nlsLexADBDo QUAS-p65AD::CaM nSyb-QF2; tub-QS; UAS-RFP; LexAop2-GFP 64347 w[*] P{y[+] w[+]=10XUAS-IVS-mCD8::RFP}attP18 P{y[+] w[+]=13XLexAop2-mCD8::GFP}su(Hw)attP8; P{y[+t7.7] w[+mC]=nSyb-MKII::nlsLexADBDo}attP24, P{w[+mC]=QUAS-p65AD::CaM}2/CyO; P{y[+t7.7] w[+mC]=nSyb-QF2.P}attP2, P{w[+mC]=tub
6 nSyb-MKII::nlsLexADBDo QUAS(FRT.stop)p65AD::CaM nSyb-QF2 61675 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::nlsLexADBDo}attP24/CyO; P{w[+mC]=QUAS(FRT.stop)p65AD::CaM}6, P{y[+t7.7] w[+mC]=nSyb-QF2.P}attP2
7 nSyb-MKIIK11A::GAL4DBDo QUAS-p65AD::CaM UAS-RFP; LexAop2-GFP; ; nSyb-QF2; tub-QS 61672 w[*] P{y[+] w[+]=10XUAS-IVS-mCD8::RFP}attP18 P{y[+] w[+]=13XLexAop2-mCD8::GFP}su(Hw)attP8; P{y[+] w[+]=nSyb-MKIIK11A::GAL4DBDo}attP24, P{w[+mC]=QUAS-p65AD::CaM}2/CyO; P{y[+] w[+]=nSyb-QF2.P}attP2, P{w[+]=tub-QS.P}9B
8 nSyb-MKIIK11A::GAL4DBDo QUAS-p65AD::CaM UAS-mCD8::GFP; nSyb-QF2; tub-QS 61673 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKIIK11A::GAL4DBDo}attP24, P{w[+mC]=QUAS-p65AD::CaM}2, P{w[+mC]=UAS-mCD8::GFP.L}LL5/CyO; P{y[+t7.7] w[+mC]=nSyb-QF2.P}attP2, P{w[+mC]=tub-QS.P}9B
Multi-component stocks with no QF construct
1 UAS-p65AD::CaM tubP-GAL80[ts 61669 w[*]; S[1]/CyO; M{w[+mC]=UAS-p65AD::CaM}ZH-86Fb, P{w[+mC]=tubP-GAL80[ts]}7
2 nSyb-MKII::GAL4DBDo QUAS-p65AD::CaM UAS-GFP 61676 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::GAL4DBDo}attP24, P{w[+mC]=QUAS-p65AD::CaM}2, P{w[+mC]=UAS-mCD8::GFP.L}LL5/CyO; Pri[1]/TM6B, Tb[1]
3 nSyb-MKII::GAL4DBDo QUAS-p65AD::CaM UAS-nSyb-GFP 61677 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::GAL4DBDo}attP24, P{w[+mC]=QUAS-p65AD::CaM}2/CyO; P{w[+mC]=UAS-nSyb-GFP.E}3
4 nSyb-MKII::GAL4DBDo QUAS-p65AD::CaM UAS-LUC 61678 w[*]; P{y[+t7.7] w[+mC]=nSyb-MKII::GAL4DBDo}attP24, P{w[+mC]=QUAS-p65AD::CaM}2/CyO; P{w[+mC]=UAS-LUC.D}3
5 nSyb-MKII::nlsLexADBDo UAS-p65AD::CaM UAS-RFP; LexAop2-GFP 61679 w[*] P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP18 P{y[+t7.7] w[+mC]=13XLexAop2-mCD8::GFP}su(Hw)attP8; P{y[+t7.7] w[+mC]=nSyb-MKII::nlsLexADBDo}attP24/CyO; M{UAS-p65AD::CaM}ZH-86Fb
6 UAS-MKII::nlsLexADBDo UAS-p65AD::CaM 62830 w[*]; P{y[+t7.7] w[+mC]=UAS-MKII::nlsLexADBDo}attP40, P{y[+t7.7] w[+mC]=UAS-p65AD::CaM}attP24/CyO; Pri[1]/TM6B, Tb[1]
7 UAS-MKII::nlsLexADBDo UAS-p65AD::CaM UAS-RFP; LexAop2-GFP 61681 w[*] P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP18 P{y[+t7.7] w[+mC]=13XLexAop2-mCD8::GFP}su(Hw)attP8; P{y[+t7.7] w[+mC]=UAS-MKII::nlsLexADBDo}attP40/CyO; M{UAS-p65AD::CaM}ZH-86Fb
8 UAS-MKII::nlsLexADBDo UAS-p65AD::CaM (2X) UAS-RFP; LexAop2-GFP 62827 w[*] P{y[+] w[+]=10XUAS-IVS-mCD8::RFP}attP18 P{y[+] w[+]=13XLexAop2-mCD8::GFP}su(Hw)attP8; P{y[+] w[+]=UAS-MKII::nlsLexADBDo}attP40, P{y[+] w[+]=UAS-p65AD::CaM}attP24/CyO; M{w[+]=UAS-p65AD::CaM}ZH-86Fb
9 UAS-MKII::nlsLexADBDo UAS-p65AD::CaM (2X) UAS-RFP; LexAop2-GFP; tubP-GAL80[ts] 62828 w[*] P{y+ w+=10XUAS-IVS-mCD8::RFP}attP18 P{y+ w+=13XLexAop2-mCD8::GFP}su(Hw)attP8; P{y+ w+=UAS-MKII::nlsLexADBDo}attP40, P{y[+] w[+]=UAS-p65AD::CaM}attP24/CyO; M{w[+]=UAS-p65AD::CaM}ZH-86Fb, P{w[+]=tubP-GAL80[ts]}7
10 nSyb-MKIIK11A::nlsLexADBDo UAS-p65AD::CaM UAS-RFP; LexAop2-GFP 61680 w[*] P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP18 P{y[+t7.7] w[+mC]=13XLexAop2-mCD8::GFP}su(Hw)attP8; P{y[+t7.7] w[+mC]=nSyb-MKIIK11A::nlsLexADBDo}attP24/CyO; M{UAS-p65AD::CaM}ZH-86Fb