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Twin spot MARCM

The following list provides stocks for uniquely marking clonal descendant cells following a mitotic recombination event. Each construct carries the gene for a tagged fluorescent protein and sequences for RNAi of a different tagged fluorescent protein. As the example in the diagram shows, no fluorescent proteins are expressed when matched pairs of constructs are present. Upon mitotic recombination and homozygosis of each construct in a different descendant cell, RNA interference is removed and fluorescent proteins are expressed.

Illustration of twin spot MARCM showing differential expression of fluors in daughter cells following mitotic recombination

These constructs were described in Awasaki et al. (2014) "Making Drosophila lineage-restricted drivers via patterned recombination in neuroblasts", Nature Neuroscience 4: 631-637.

For most chromosome arms, basal FRT insertions have not yet been combined with the twin spot MARCM insertions.

  Stk # Promoter Fluor RNAi Arm Genotype
1 56176 lexAop CD2.RFP GFP 2L w[*]; P{y[+t7.7] w[+mC]=lexAop-rCD2.RFP.lexAop-GFPi}attP40/CyO
2 56177 lexAop CD2.RFP GFP 2R w[*]; P{y[+t7.7] w[+mC]=lexAop-rCD2.RFP.lexAop-GFPi}su(Hw)attP5/CyO
3 56175 lexAop CD2.RFP GFP 3L w[*]; P{y[+t7.7] w[+mC]=lexAop-rCD2.RFP.lexAop-GFPi}attP2
4 56174 lexAop GFP CD2 2L w[*]; P{y[+t7.7] w[+mC]=lexAop-mCD8.GFP.lexAop-rCD2i}attP40
5 56750 lexAop GFP CD2 2R w[*]; P{y[+t7.7] w[+mC]=lexAop-mCD8.GFP.lexAop-rCD2i}su(Hw)attP5
6 56173 lexAop GFP CD2 3L w[*]; P{y[+t7.7] w[+mC]=lexAop-mCD8.GFP.lexAop-rCD2i}attP2
7 56182 lexAop GFP CD2 3R w[*]; PBac{y[+mDint2] w[+mC]=lexAop-mCD8.GFP.lexAop-rCD2i}VK00027
8 56183 lexAop GFP CD2 3R w[*]; PBac{y[+mDint2] w[+mC]=lexAop-rCD2.RFP.lexAop-GFPi}VK00027
9 56181 UAS CD2.RFP GFP 2L w[*]; P{y[+t7.7] w[+mC]=UAS-rCD2.RFP.UAS-GFPi}attP40
10 56184 UAS CD2.RFP GFP 2L y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-rCD2.RFP.UAS-GFPi}attP40 P{ry[+t7.2]=neoFRT}40A/CyO; TM3, Sb[1]/TM6B, Tb[1]
11 56180 UAS CD2.RFP GFP 2R w[*]; P{y[+t7.7] w[+mC]=UAS-rCD2.RFP.UAS-GFPi}su(Hw)attP5
12 56179 UAS CD2.RFP GFP 3L w[*]; P{y[+t7.7] w[+mC]=UAS-rCD2.RFP.UAS-GFPi}attP2
13 56178 UAS CD2.RFP GFP 3R w[*]; PBac{y[+mDint2] w[+mC]=UAS-rCD2.RFP.UAS-GFPi}VK00027
14 56172 UAS GFP CD2 2L w[*]; P{y[+t7.7] w[+mC]=UAS-mCD8.GFP.UAS-rCD2i}attP40
15 56185 UAS GFP CD2 2L y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-mCD8.GFP.UAS-rCD2i}attP40 P{ry[+t7.2]=neoFRT}40A/CyO; TM3, Sb[1]/TM6B, Tb[1]
16 56171 UAS GFP CD2 2R w[*]; P{y[+t7.7] w[+mC]=UAS-mCD8.GFP.UAS-rCD2i}su(Hw)attP5
17 56170 UAS GFP CD2 3L w[*]; P{y[+t7.7] w[+mC]=UAS-mCD8.GFP.UAS-rCD2i}attP2
18 56169 UAS GFP CD2 3R w[*]; PBac{y[+mDint2] w[+mC]=UAS-mCD8.GFP.UAS-rCD2i}VK00027