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UAS constructs for mosquito odorant receptors

We have two sets of stocks that allow mosquito odorant receptors to be expressed in Drosophila under UAS control.

The UAS constructs are maintained with the aberrations Df(2L)dp-79b and Dp(2;2)dpp[d21], which together create a synthetic deletion for the Or22a and Or22b genes (commonly called “Delta halo” flies). Antennal olfactory receptor neuron ab3A loses its odorant response in flies lacking these genes. This “empty neuron” allows the function of any odorant receptor to be tested in the absence of the naturally occurring odorant receptors.

The “empty neuron” system was described in Dobritsa et al. (2003) “Integrating the molecular and cellular basis of odor coding in the Drosophila antenna” Neuron 37: 827–841.

Go to the UAS home page.

In the first set of stocks, seven allelic variants of Odorant receptor 4 from Aedes aegypti are expressed under UAS control. Mosquitoes differ in their preference for humans over other animals depending on the Or4 alleles they carry.

These stocks were described in McBride et al. (2014) “Evolution of mosquito preference for humans linked to an odorant receptor” Nature 515: 222–227.

Stk # Gene Symbol Genotype
56143 Or4, isoform A (Aedes) M{UAS-AaegOr4.A}ZH-86Fb w[1118]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; M{w[+mC]=UAS-AaegOr4.A}ZH-86Fb
56144 Or4, isoform B (Aedes) M{UAS-AaegOr4.B}ZH-86Fb w[1118]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; M{w[+mC]=UAS-AaegOr4.B}ZH-86Fb
56145 Or4, isoform C (Aedes) M{UAS-AaegOr4.C}ZH-86Fb w[1118]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; M{w[+mC]=UAS-AaegOr4.C}ZH-86Fb
56146 Or4, isoform D (Aedes) M{UAS-AaegOr4.D}ZH-86Fb w[1118]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; M{w[+mC]=UAS-AaegOr4.D}ZH-86Fb
56147 Or4, isoform E (Aedes) M{UAS-AaegOr4.E}ZH-86Fb w[1118]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; M{w[+mC]=UAS-AaegOr4.E}ZH-86Fb
56148 Or4, isoform F (Aedes) M{UAS-AaegOr4.F}ZH-86Fb w[1118]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; M{w[+mC]=UAS-AaegOr4.F}ZH-86Fb
56149 Or4, isoform G (Aedes) M{UAS-AaegOr4.G}ZH-86Fb w[1118]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; M{w[+mC]=UAS-AaegOr4.G}ZH-86Fb
 
The second set of stocks carry UAS constructs for Anopheles gambiae odorant receptors. The stocks were described in Carey et al. (2010) "Odorant reception in the malaria mosquito Anopheles gambiae" Nature 464: 66–71.
Stk # Gene Symbol Genotype
58827 Or1 (Anopheles) P{UAS-AgamOr1}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr1}3
58835 Or10 (Anopheles) P{UAS-AgamOr10}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr10}3
58836 Or11 (Anopheles) P{UAS-AgamOr11}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr11}3/TM3, Sb[1]
58837 Or12 (Anopheles) P{UAS-AgamOr12}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr12}3
58838 Or13 (Anopheles) P{UAS-AgamOr13}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr13}3
58839 Or15 (Anopheles) P{UAS-AgamOr15}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr15}3
58840 Or16 (Anopheles) P{UAS-AgamOr16}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr16}3
58841 Or18 (Anopheles) P{UAS-AgamOr18}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr18}3
58828 Or2 (Anopheles) P{UAS-AgamOr2}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr2}3/TM3, Sb[1]
58842 Or20 (Anopheles) P{UAS-AgamOr20}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr20}3
58843 Or21 (Anopheles) P{UAS-AgamOr21}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr21}3
58844 Or23 (Anopheles) P{UAS-AgamOr23}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr23}3
58845 Or25 (Anopheles) P{UAS-AgamOr25}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr25}3
58846 Or26 (Anopheles) P{UAS-AgamOr26}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr26}3
58847 Or27 (Anopheles) P{UAS-AgamOr27}2 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21], P{w[+mC]=UAS-AgamOr27}2/CyO; Dr[1]/TM3, Sb[+]
58829 Or3 (Anopheles) P{UAS-AgamOr3}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr3}3
58848 Or30 (Anopheles) P{UAS-AgamOr30}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr30}3
58849 Or31 (Anopheles) P{UAS-AgamOr31}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr31}3
58850 Or32 (Anopheles) P{UAS-AgamOr32}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr32}3
58851 Or33 (Anopheles) P{UAS-AgamOr33}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr33}3
58852 Or35 (Anopheles) P{UAS-AgamOr35}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr35}3
58853 Or38 (Anopheles) P{UAS-AgamOr38}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr38}3
58854 Or39 (Anopheles) P{UAS-AgamOr39}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr39}3
58830 Or4 (Anopheles) P{UAS-AgamOr4}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr4}3
58855 Or41 (Anopheles) P{UAS-AgamOr41}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr41}3
58856 Or42 (Anopheles) P{UAS-AgamOr42}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr42}3
58857 Or43 (Anopheles) P{UAS-AgamOr43}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr43}3
58858 Or44 (Anopheles) P{UAS-AgamOr44}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr44}3
58859 Or45 (Anopheles) P{UAS-AgamOr45}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr45}3
58860 Or46 (Anopheles) P{UAS-AgamOr46}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr46}3
58861 Or48 (Anopheles) P{UAS-AgamOr48}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr48}3
58831 Or5 (Anopheles) P{UAS-AgamOr5}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr5}3
58862 Or50 (Anopheles) P{UAS-AgamOr50}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr50}3
58863 Or53 (Anopheles) P{UAS-Myc-AgamOr53}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-Myc-AgamOr53}3/TM3, Sb[1]
58864 Or54 (Anopheles) P{UAS-AgamOr54}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr54}3
58865 Or56 (Anopheles) P{UAS-AgamOr56}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr56}3
58866 Or57 (Anopheles) P{UAS-AgamOr57}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr57}3
58867 Or59 (Anopheles) P{UAS-AgamOr59}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr59}3
58832 Or6 (Anopheles) P{UAS-AgamOr6}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr6}3
58868 Or61 (Anopheles) P{UAS-AgamOr61}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr61}3
58869 Or63 (Anopheles) P{UAS-AgamOr63}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr63}3
58870 Or64 (Anopheles) P{UAS-AgamOr64}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr64}3
58871 Or65 (Anopheles) P{UAS-AgamOr65}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr65}3
58872 Or66 (Anopheles) P{UAS-AgamOr66}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr66}3
58873 Or67 (Anopheles) P{UAS-AgamOr67}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr67}3
58874 Or73 (Anopheles) P{UAS-AgamOr73}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr73}3/TM3, Sb[1]
58875 Or75 (Anopheles) P{UAS-AgamOr75}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr75}3
58876 Or76 (Anopheles) P{UAS-AgamOr76}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr76}3/TM3, Sb[1]
58833 Or8 (Anopheles) P{UAS-AgamOr8}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr8}3
58834 Or9 (Anopheles) P{UAS-AgamOr9}3 w[*]; Df(2L)dp-79b Dp(2;2)dpp[d21]/CyO; P{w[+mC]=UAS-AgamOr9}3