Sucrose and Yeast Medium in use at the Neurosciences Institute
Updated March 30, 2007
Stock Center logo
This recipe has not been used at Bloomington.
The recipe
Water (distilled) 1 liter
Agar* 10 grams
Sucrose 15 grams
Yeast 35 grams
Light corn syrup (Karo) 30 milliliters
15% p-Hydroxy-benzoic acid methyl ester in 95% ethanol 5 milliliters
Propionic acid 7.5 milliliters
Penicillin-streptomycin soln. 1 milliliter
*adjust agar concentration according to the gel strength of your agar