If you wish to import transgenic Drosophila strains, you must apply for an import permit through Biotechnology Regulatory Services (BRS).

BRS has switched to online permit applications, so we are now providing step-by-step instructions for the USDA “e-permits” system. To use the e-permits system, you must register online.

We formerly provided a partially completed version of a paper application called Form 2000, which we had customized for Drosophila, but BRS will no longer accept our version of Form 2000 at the end of 2007. We strongly recommend that you use the new e-permits system. Applications submitted electronically will receive expedited review.

BRS issues different permits for transgenic Drosophila carrying DNA sequences from plant pests and for transgenic Drosophila lacking plant pest sequences (see Federal Genetically-Engineered Plant Pest Regulations). We provide guidance in choosing the appropriate application form at How do I know if my construct carries sequences from a plant pest?

Most Drosophila constructs do not carry plant pest sequences; consequently, most researchers will use the directions at Applying for a permit to import flies with routine transgenic constructs lacking plant pest sequences. 

For those few transgenic strains carrying plant pest sequences, we provide application instructions at Applying for a permit to import flies carrying transgenic constructs with sequences from plant pests.

If you have already received an import permit and it has expired, you can renew the permit online.