The FlyLight Project at Janelia has generated a set of stocks carrying combinations of "split" transcriptional activators that activate UAS reporters in specific sets of neurons in the adult mushroom body and optic lobe. Another set of Janelia-based stocks has been characterized by Marta Zlatic's group for expression in larval neural circuits.
The expression patterns for FlyLight-characterized lines can be found at the Janelia FlyLight site and the Virtual Fly Brain (VFB) site. Links to the data for individual lines are provided in the FlyLight and VFB columns.
See the Janelia FlyLight site and Meissner et al. (2023) A split-GAL4 driver line resource for Drosophila CNS cell types for more information and references.
Control stocks - These stocks carry the GAL4 DNA binding domain and/or the p65 activation domain in the same vector used to make the Janelia split GAL4 collection but with no regulatory fragment present.
| Stk # | Genotype | Also known as |
| 71209 | w[1118]; P{GAL4-DBD.Uw}attP2 | 'GAL4-DBD.empty' control, BPZpGDBD or pBPZpGAL4DBDUw (attP2). |
| 71210 | w[1118]; P{p65-AD.Uw}attP40 | 'p65-AD.empty' control, BPp65ADZp or pBPp65ADZpUw (attP40). |
| 79603 | w[1118]; P{p65-AD.Uw}attP40; P{GAL4-DBD.Uw}attP2 | 'empty stable split' control |
For other stocks expressing both an activation and DNA-binding domain see:
- Transcription-based calcium sensors
For stocks expressing either an activation OR a DNA-binding domain see:
- Split transcriptional activator page