Lineage and Clonal Analysis

Brief descriptions and links to pages for lineage and clonal analysis system stocks available at the BDSC are shown here (in alphabetical order). The pictures are examples and may not represent all that is available. See relevant pages for all the possibilities for each system.

BitbowBrainbowCoinFLP
DFS (female germline)Double MARCM (DMARCM)FlyBow
G-TRACE and i-TRACEIntMEMOIRLineage filtering
MARCMMultiColor FlpOutPhotoreceptor clones
RaeppliTwin spot MARCMTie-Dye
Bitbow
Stochastic FLP-mediated inversion within matched FRT cassettes result in cells marked with one or more possible marker combinations.
       
Brainbow
Stochastic cre-mediated flip-outs between matched lox sites result in clones marked with one of several possible marker combinations.
       
 
FLP-out of one of two overlapping FRT or FRT3 stop cassettes results in GAL4- or lexA-expressing clones.
        CoinFLP
 
Dominant Female Sterile (DFS) stocks for making female germline clones
Stocks for assessing gene function in the female germ line by clonal analysis using "ovo[D]" stocks.
DFS
Double MARCM (DMARCM)
Low-frequency labeling of cell lineages that is linearly scalable with heat shock duration.
        dmarcm
 
FlyBow.1.0 and FlyBow.2.0
Stochastic FLP-mediated inversions and/or FLP-outs result in clones marked with one of several possible marker combinations.
        flybow
 
G-TRACE (GAL4 Technique for Real-time And Clonal Expression)
Stochastic FLP-out of a stop cassette marks a clone with a fluor.
G-TRACE
 
IntMEMOIR
10 unique barcodes are stochastically deleted or inverted to mark cell lineages
schematic of the 10 bxb1 target site-flanked barcodes that make up the intmemoir system
 
Lineage filtering
FLP-out cassettes are used to restrict ('filter') expression to the descendents of specific cells.
Lineage filtering
 
 
Photoreceptor clones
FLP-mediated recombination results in photoreceptor clones marked with one of two fluors.
        Tomato/GFP-FLP/FRT
Raeppli
FLP-out of a stop cassette results in phiC31 integrase expression. Subsequent stochastic attP-based rearrangements result in expression of fluors under UAS or lexA control. Resulting clones can be marked with one of ten possible color combinations.
        Raeppli
 
Tie-DYE
Stochastic FLP-out of a stop cassette from three constructs results in clones marked with one of seven possible marker combinations plus/minus a gene of your choice.
        Tie-DYE
 
 
Twin spot MARCM
Mitotic recombination separates a fluor from the RNAi that targets it, resulting in clonal expression.
        twin_spot
 

Related links   Fluors   FRT   FLP