The MiMIC collection, generated by the Gene Disruption Project, is composed of a large set of Minos-based insertions carrying an attP-flanked gene trap cassette. Recombinase-Mediated Cassette Exchange (RMCE) can be used to swap the gene trap cassette with attB-flanked cassettes of your choice using phiC31 integrase. DNA carrying attB-flanked donor cassettes can be supplied either by injection or via a "donor" transgene using genetic crosses (Venken et al., 2011; Nagarkar-Jaiswal et al., 2015). ![]()
The following are links to pages with stocks carrying donor transgenes that can be used to carry out swaps genetically. More information and references can be found on each page.
| Donors for making: | Brief Description |
|---|---|
| Protein traps | Donors for making tagged proteins using MiMIC insertions inside coding introns. |
| GAL4 lines, Trojan | Donors for expressing GAL4 in the pattern of a gene using intronic MiMIC insertions. |
| Double Header traps | Donors for making either tagged proteins or T2A-GAL4 gene traps depending on the orientation of the swap. |